Table 4. Peptide profile by LC-MS

Step	Total time (min)	Flow rate (μL/min)	A (%)	B (%)
0	0	200.00	95.0	5.0
1	5	200.00	95.0	5.0
2	28	200.00	70.0	30.0
3	33	200.00	5.0	95.0
4	40	200.00	5.0	95.0
5	41	200.00	95.0	5.0
6	46	200.00	95.0	5.0

LC-MS, liquid chromatography-mass spectrometry.