Table 5. Comparison of the Ct value of E. sakazakii (Cronobacter spp.) and non-E. sakazakii (non-Cronobacter spp.) using by various real-time PCR machine

Type of real-time PCR machine	List of stains tested in this study (Ct value)										Volume of format
	Escherichia vulneris	Enterobacter cloacae	E. sakazakii (Cronobacter spp.)				Salmonella Enteritidis	Water	Positive control	Negative control
	Escherichia vulneris	Enterobacter cloacae	ES626	N6	FSM282	N6	Salmonella Enteritidis	Water	Positive control	Negative control
ABI Prism 7000 SDS	Undet¹⁾	Undet	24.88	25.15	20.90	24.05	Undet	Undet	22.49	Undet	50 µL
Rotor-gene	Undet	Undet	17.03	23.59	15.90	17.25	Undet	Undet	15.76	Undet	25 µL
Smart cycler	Undet	Undet	18.33	23.23	17.22	19.55	Undet	Undet	17.59	Undet	25 µL
Light cycler	Undet	Undet	Undet	Undet	Undet	Undet	Undet	Undet	Undet	Undet	20 µL

undetection.