Table 4. Detection limit of E. sakazakii (Cronobacter spp.) N6 between DFI and real-time PCR after 40 mL sample spin down
Inoculation concentration | E. sakazakii (Cronobacter spp.) N6 |
40 mL spin down (log cfu/mL) | P/T1) |
6 h | 24 h | 6 h | 24 h |
56 cfu/30 g | 5.78 | 9.83 | 2/2 | 2/2 |
7 cfu/30 g | 5.22 | 10 | 2/2 | 2/2 |
2 cfu/30 g | 5.05 | 10 | 2/2 | 2/2 |
0 cfu/30 g | - | - | 0/2 | 0/2 |
Positive number per total number analyzed by real-time PCR.
DFI, Druggan-Forsythe-Iversen.