Table 2. Operating conditions of UHPLC for analyzing organic acids
Specification
Condition
Column
HYPERSSIL GOLD AQ C
18
(5 μm, 250×4.6 mm), Thermo Fisher
Detector
DAD detector (210 nm)
Mobile phase
20 mM Na
2
SO
4
Flow rate
0.8 mL/min
Column temperature
40°C
Injection volume
10
μ
L
Run time
20 min