| Items | Conditions |
|---|---|
| Instrument | Agilent 1200 HPLC ChemStationTM(Agilent, USA) |
| Column | Guard Column(4.6 mm ID × 12.5 mm, Agilent, USA)Hi-Plex Ca Carbohydrate Analysis column(6.5 mm×300 mm) |
| Detector | 210 and 290 nm UV Detector (325 Dual Wavelength UV-Vis Detector, Agilent, USA)Refractive Index Detector(RID, Agilent, USA) |
| Mobile phase | A: H2O |
| Flow rate | 0.6 mL/min |
| Column temp. | 80℃ |
| Injection volume | 10 uL |