Table 4 The result of concentration range setting test conducted to evaluate the effect on increase & decrease of reverse mutation colonies by the addition of 23%-GNANA, a test substance, under the presence and absence conditions of metabolic activation system

With and without metabolic activation		Test materials conc.(μg/plate)	Number of colony (cfu/plate)
			Base substitution types					Frameshift types
			TA100*	TA1535*		WP2uvrA*		TA98*	TA1537*
S9Mix(－)		0#	102.3±8.08#	31.7±2.08#		32.7±4.62#		46.7±4.73#	13.3±1.53#
		312	108.3±6.81	33.7±0.58		36.7±1.15		50.7±3.06	14.0±1.00
		625	109.0±9.54	37.3±2.08		34.7±4.16		51.7±4.73	14.3±2.08
		1,250	115.0±3.00	35.7±4.16		36.0±0.00		51.7±2.31	11.7±3.06
		2,500	112.3±3.51	32.3±1.53		36.0±4.36		50.3±3.06	11.3±2.08
		5,000	124.3±18.88	32.3±3.06		35.3±3.06		49.0±1.00	12.3±3.51
S9Mix(+)		0#	144.7±3.79	33.7±2.52		35.3±3.06		52.7±3.21	13.3±0.58
		312	147.3±12.66	37.0±2.65		32.7±2.31		48.0±3.61	13.0±2.00
		625	147.0±9.17	33.3±4.04		34.0±5.29		49.0±4.36	11.7±0.58
		1,250	139.7±8.33	31.7±2.52		32.0±4.00		51.0±3.00	15.7±1.53
		2,500	144.0±5.00	33.7±3.21		32.0±2.00		50.3±3.06	12.7±2.08
		5,000	150.0±6.56	32.3±3.21		31.3±4.16		49.3±3.79	15.3±2.08
Positive control	S9Mix(－)	Materials	NaN3	NaN3		4-NQO		4-NQO	9-AA
		Conc. (μg/plate)	1.5	1.5		0.5		0.5	80
		cfu/plate	357.7±9.61	159.3±8.62		260.0±24.98		252.7±4.93	136.0±7.00
	S9Mix(+)	Materials	2-AA	2-AA		2-AA		2-AA	2-AA
		Conc. (μg/plate)	1.0	2.0		10		0.5	2.0
		cfu/plate	372.7±13.01	160.0±22.72		283.3±20.03		259.3±22.48	204.7±15.31

^*: TA98 (Sal. typhimurium TA98), TA100 (Sal. typhimurium TA100), TA1535 (Sal. typhimurium TA1535), TA1537 (Sal. typhimurium TA1537), WP2uvrA (E. coli WP2uvrA), ^#: Represents a significant difference at p<0.01 level compared with the vehicle control