Table 1 HPLC operation conditions for analysis of sialic acid

HPLC system(Agilent 1260)	2. Analysis conditions
․Pump: G1311C ․Auto sampler:G1329B ․Column:G1316A ․UV detector:G1314F	․Wavelength: 205 nm ․Column: Aminex® HPX-87H Ion Exclusion Column (300×7.8 mm, 9 μm, Bio-Rad Co.) ․Mobile phase: 10 mM H2SO4 ․Running time: 20 min․Flow rate: 0.5 mL/min ․Injection volume: 10 μL ․Temperature: 40℃ ․Standard sol.(w/w): Silaic acid 0.1 ppm, 1 ppm, 10 ppm